NOW AVAILABLE
- Meets NEW FDA/UVA in-vitro test procedure
- Now with NEW Validation Kit
- NEW software v4.0
- Windows® automation and measurement software
- Supports revised Boots protocol
OVER 300 CUSTOMERS WORLDWIDE
Avon, BASF, Clairol, Elizabeth Arden, Estee Lauder, Good Housekeeping, ISP, Johnson & Johnson, Lever Bros, L’Oreal, Mary Kay, Merck, Revlon, Rohm & Haas, Proctor & Gamble, Unilever, Andrew Jergens, Helene Curtis, Whitehall Robins, Bayer, S/C/Johnson, Bristol-Myers Squibb, Tanning Research, Pfizer, Boots (the Chemist) and Amway…to name just a few!
Setting the standard for in-vitro SPF assays of:
- Lotions
- Creams
- Cosmetics
- Fabrics
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SOFTWARE FEATURES
- Calculate and print SPF values
- Both tabular and graphical formats
- Boots Star Ratings calculated
- Create formulation assays of up to 36 scans
- Display MPF and absorbance values
- Photo-stability testing
- Calculate area under the curve
- Critical wavelength calculation
- UVA/UVB ratio
- Complete on-line help menu
- View scans individually
- Calculates TNUV standard
- Supports FDA UVA in-vitro test procedure
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AND NEW WITH WinSPF v4.0
- Ability to use wavelength intervals of 1, 2 or 5nm, supporting revised Boots protocol
- Support for up to 12 reference scans to eliminate variations in reference measurements
- Taylor approximation for calculating MPF standard deviations with multiple reference scans
- Optional solar simulator spectra
- Factors out dark current
- Trapezoidal or Simpson’s rule approximations for integral calculations
- New comprehensive test feature for use with Validation Kit
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CORRELATION BETWEEN SPF-290S AND IN-VIVO SPF TESTING
The high correlation between the SPF-290S’s in-vitro measurements and in-vivo test results gives you confidence that the instrument will be a reliable guide to product performance.
The chart shows data comparisons for a variety of commercially available products including:
- physical sunscreens
- sprays
- waterproof sunscreens and stick formulations
The SPF-290S can provide reliable results on the most difficult samples.
An easy-to-use testing methodology combined with reproducible results opens the door to efficient and low cost experimental design techniques for formulation optimization. This will result in faster formulation and lower development cost due to a reduction in the need for extensive in-vivo panel studies.

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MEASUREMENT
The SPF-290S is a recording UV spectrophotometer designed and optimized for the determination of SPF values on a variety of sunscreen and cosmetic products reducing the need (and cost!) for in-vivo testing.
Covering both the UVB and UVA spectral regions, the system automatically scans from 290 to 400 nm, accumulating and storing data at intervals of 1, 2 or 5nm. The monochromatic protection factor (MPF) is determined for each of the selected wavelengths and is used to calculate the SPF value, using solar irradiance and erythemal constants that are programmed into the software but which can be easily modified.

SPF-290S SAMPLE HANDLING & Support media
Liquids, creams and gels are applied in small “dabs” or “spots” to the Transpore Tape® or other substrate, with the pipette supplied with the system. The substrate is placed on an open metal frame. The sample is spread lightly and evenly over a 50 cm2 area at 2 μl/cm2, equivalent to in-vivo testing.
Quartz plates are available from Optometrics for sprays and other difficult samples.
COMPUTER CONTROLLED SAMPLING STAGE
There are two modes of computer controlled operation:
- programmed reading of up to 12 locations (Autoscan), and,
- time based measurements (photo-stability)
Autoscan mode offers two methods of choosing up to 12 sampling locations. The operator can either specify the positions to be read or the computer can generate them randomly. Once set, the operation, data collection and reporting are performed automatically. The stage moves the sample on the holder into the light beam, takes the measurements, moves to the next position and continues until all the measurements have been completed.
Time-based (photo-stability) measurements monitor the SPF values for a sample at a user specified position against time. The effects of drying and exposure to air and light on a sample can be easily evaluated, making it particularly suitable for photostability testing. The system’s computer provides a controlled repeatable time base for measurements.
Computation of statistics for multiple assays is provided by the software.

X-Y SAMPLING STAGE The Computer Controlled X-Y Sampling Stage:
- Reduces the overall cost of testing,
- Frees technicians for other tasks,
- Improves the repeatability and accuracy of
measurements, and - Facilitates photo-stability testing
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| SPF-290S SPECIFICATIONS |
| Source: |
| Lamp |
Xenon CW |
| Power |
125 W (operated at 75 W) |
| Detector: |
| Type |
Multialkali side-on PMT |
| Dimensions: |
| System Dimensions |
46 x 32 x 35 cm |
| Monochromator: |
| Resolution |
1.66 nm |
| Wavelength Accuracy |
± 0.2% |
| System Spectral Range |
290 nm to 400 nm |
| Wavelength Reproducibility |
0.25 nm | |
WinSPF VERSION 4.0 SOFTWARE In August of 2007 the US Department of Health and Human Services, Food and Drug Administration proposed a UVA in-vitro testing procedure. The measurement takes the ratio of UVA 1 area per unit wavelength to UV area per unit wavelength.
WinSPF Release 4.0 performs the calculations and ratings prescribed in the Federal Register thus allowing the SPF-290S user to evaluate their products to ensure they conform to US Federal labeling guidelines.
Beginning with WinSPF Release 3.0, Optometrics software added the capability to make more than a single reference measurement, allowing up to 12 spatially separated measurements of the blank sample. With both the Boots Star rating and the new proposed Federal rules calling for the use of Taylor’s approximation for MPF and transmittance standard deviation reporting, WinSPF Release 4.0 calculates all MPF and transmittance standard deviations using this method when multiple references recipes are used. When a single reference scan is prescribed, WinSPF 4.0 performs the standard deviation evaluation in the more familiar manner. |
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Turnkey Basic System
SPF-290S Sunscreen Protection Factor Analyzer System and computer comprised of a 125W CW Xenon source, integrating sphere, color compensating filter, three sample holders, scanning monochromator with stepping motor and controller, in a self-contained, attractive desktop unit. Includes data acquisition board and drivers, I/O, RS-232 and power cables and WinSPF operating software (on 3.5" floppy disks), four rolls of Transpore® tape (8-5010), a box of 25 syringes (8-5015), two ND calibration filters (1.0 and 1.5 OD) and complete instruction manual. WinSPF operating software also includes complete on-line help system, fully functional toolbar, single step control icons, graphical and tabular data display formats and utilizes all Windows® multi-tasking features. Available for use with 110V AC, 60 Hz. (catalog no. 8-5002) or 220V AC, 50 Hz. (catalog no. 8-5003). Computer is a Pentium IV (or better) with 64 MB (or better) RAM, 40 GB hard drive, keyboard, monitor, mouse, Windows XP Pro operating system; Data acquisition board, drivers and Win SPF software installed and tested.
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$17,500 Annual SPF analyzer service contract available after first year for $2,095; Contact sales for detail on contract benefits. |
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Turnkey Automated System
SPF-290S Sunscreen Protection Factor Analyzer System and computer comprised of a 125W CW Xenon source, integrating sphere, color compensating filter, six sample holders, scanning monochromator with stepping motor and controller, X-Y Module and electronic X-Y Control Module, in a self-contained, attractive desktop unit. Includes data acquisition board and drivers, I/O, RS-232 and power cables and WinSPF operating software (on 3.5" floppy disks), four rolls of Transpore® tape (8-5010), a box of 25 syringes (8-5015), two ND calibration filters (1.0 and 1.5 OD) and complete instruction manual. WinSPF operating software also includes complete online help system, fully functional toolbar, single step control icons, graphical and tabular data display formats and utilizes all Windows® multi-tasking features. Available for use with 110V AC, 60 Hz. (catalog no. 8-5005) or 220V AC, 50 Hz. (catalog no. 8-5006). Computer is a Pentium IV (or better) with 64 MB (or better) RAM, 40 GB hard drive, keyboard, monitor, mouse, Windows XP Pro operating system; Data acquisition board, drivers and Win SPF software installed and tested.
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$22,200 Annual SPF Analyzer service contract available after first year for $2,350; Contact sales for detail on contract benefits. |
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Technical Application Notes
Application Note Number 1
Application Note Number 2
Application Note Number 3
Application Note Number 4
Application Note Number 5
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