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NOW AVAILABLE
- New Software v4.1
- Meets new FDA 21CFR
- Meets Colipa UVA Guidelines. Includes spreadsheet
- Supports two international fabric standards
- Includes Validation Kit
- NEW Audio Visual Training Aids
- NEW Accessories
OVER 300 CUSTOMERS WORLDWIDE
Avon, BASF, Clairol, Elizabeth Arden, Estee Lauder, Good Housekeeping, ISP, Johnson & Johnson, Lever Bros, L’Oreal, Mary Kay, Merck, Revlon, Rohm & Haas, Proctor & Gamble, Unilever, Andrew Jergens, Helene Curtis, Whitehall Robins, Bayer, S/C/Johnson, Bristol-Myers Squibb, Tanning Research, Pfizer, Boots (the Chemist) and Amway…to name just a few!
Setting the standard for in-vitro SPF assays of:
- Lotions
- Creams
- Cosmetics
- Fabrics
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SOFTWARE FEATURES
- Critical wavelength calculation
- Calculate and print SPF values
- Both tabular and graphical formats
- Boots Star Ratings calculated
- Create formulation assays of up to 36 scans
- Display MPF and absorbance values
- Photo-stability testing
- Calculate area under the curve
- UVA/UVB ratio
- Complete on-line help menu
- View scans individually
- Calculates TNUV standard
- Supports FDA UVA in-vitro test procedure
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AND NEW WITH WinSPF v4.1
- Meets FDA 21 CFR
- Meets and includes spreadsheet for Colipa UVA Guidelines
- AVTA - Audio Visual Training Aids
- New sample pattern for PMMA plates
- Updated help system
- Compliant with fabric standards AATCC-183 and AS NZS 4399
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CORRELATION BETWEEN SPF-290S AND IN-VIVO SPF TESTING The high correlation between the SPF-290S’s in-vitro measurements and in-vivo test results gives you confidence that the instrument will be a reliable guide to product performance.
The chart shows data comparisons for a variety of commercially available products including:
- physical sunscreens
- sprays
- waterproof sunscreens and stick formulations
The SPF-290S can provide reliable results on the most difficult samples.
An easy-to-use testing methodology combined with reproducible results opens the door to efficient and low-cost experimental design techniques for formulation optimization. This will result in faster formulation and lower development cost due to a reduction in the need for extensive in-vivo panel studies.

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MEASUREMENT The SPF-290S is a recording UV spectrophotometer designed and optimized for the determination of SPF values on a variety of sunscreen and cosmetic products reducing the need (and cost!) for in-vivo testing.
Covering both the UVB and UVA spectral regions, the system automatically scans from 290 to 400 nm, accumulating and storing data at intervals of 1, 2 or 5nm. The monochromatic protection factor (MPF) is determined for each of the selected wavelengths and is used to calculate the SPF value, using solar irradiance and erythemal constants that are programmed into the software but which can be easily modified.

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SPF-290S SAMPLE HANDLING & SUPPORT MEDIA
Liquids, creams and gels are applied in small “dabs” or “spots” to the Transpore Tape® or other substrate, with the pipette supplied with the system. The substrate is placed on an open metal frame. The sample is spread lightly and evenly over a 50 cm2 area at 2 μl/cm2, equivalent to in-vivo testing.
PMMA and quartz plates are available from Optometrics.
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COMPUTER-CONTROLLED SAMPLING STAGE There are two modes of computer-controlled operation:
- programmed reading of up to 12 locations (Autoscan), and,
- time-based measurements (photo-stability)
Autoscan mode offers two methods of choosing up to 12 sampling locations. The operator can either specify the positions to be read or the computer can generate them randomly. Once set, the operation, data collection and reporting are performed automatically. The stage moves the sample on the holder into the light beam, takes the measurements, moves to the next position and continues until all the measurements have been completed.
Time-based (photo-stability) measurements monitor the SPF values for a sample at a user-specified position against time. The effects of drying and exposure to air and light on a sample can be easily evaluated, making it particularly suitable for photostability testing. The system’s computer provides a controlled repeatable time base for measurements.
Computation of statistics for multiple assays is provided by the software.

The Computer-Controlled X-Y Sampling Stage:
- Reduces the overall cost of testing,
- Frees technicians for other tasks,
- Improves the repeatability and accuracy of
measurements, and - Facilitates photo-stability testing
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| SPF-290S SPECIFICATIONS |
| Source: |
| Lamp |
Xenon CW |
| Power |
125 W (operated at 75 W) |
| Detector: |
| Type |
Multialkali side-on PMT |
| Dimensions: |
| System Dimensions |
46 x 32 x 35 cm |
| Monochromator: |
| Resolution |
1.66 nm |
| Wavelength Accuracy |
± 0.2% |
| System Spectral Range |
290 nm to 400 nm |
| Wavelength Reproducibility |
0.25 nm | |
WinSPF VERSION 4.1 SOFTWARE
As the FDA has recently published 21 CFR, Optometrics has kept pace with the release of WinSPF v4.1. It incorporates a new (X-Y Stage) sampling pattern for use with PMMA plates as well as the critical wavelength calulation as delineated.
Many of the changes to the monograph are in an effort to harmonize it with the Colipa Guidelines, which WinSPF also supports. The prescribed spreadsheet is included to calculate all needed results.
In addition, v4.1 now meets the requirements of two international fabric standards: AATCC-183 and AS NZS 4399.
As an augmentation to the help system, the software now comes with Audio Visual Traningin Aids designed to facilitate ease of use, routine maintenance, and sample preparation, et. al.
In August of 2007 the US Department of Health and Human Services, Food and Drug Administration proposed a UVA in-vitro testing procedure. The measurement takes the ratio of UVA 1 area per unit wavelength to UV area per unit wavelength.
Beginning with Release 4.0 WinSPF performs the calculations and ratings prescribed in the Federal Register thus allowing the SPF-290S user to evaluate their products to ensure they conform to US Federal labeling guidelines. |
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Turnkey Basic System
SPF-290S Sunscreen Protection Factor Analyzer System and computer comprised of a 125W CW Xenon source, integrating sphere, color-compensating filter, three sample holders, scanning monochromator with stepping motor and controller, in a self-contained, attractive desktop unit. Includes data acquisition board and drivers, I/O, RS-232 and power cables and WinSPF operating software, four rolls of Transpore® tape (8-5010), a box of 25 syringes (8-5015), validation kit and complete instruction manual. WinSPF operating software also includes Audio Visual Training aids, Colipa spreadsheet, complete on-line help system, fully functional toolbar, single-step control icons, graphical and tabular data display formats and utilizes all Windows® multi-tasking features. Available for use with 110V AC, 60 Hz. (catalog no. 8-5002) or 220V AC, 50 Hz. (catalog no. 8-5003). Computer will be a current Windows® model capable of running WinSPF software and will include keyboard, monitor, mouse. Data acquisition board, drivers and Win SPF software installed and tested.
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$17,500 Annual SPF analyzer service contract available after first year for $2,095; Contact sales for detail on contract benefits. |
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Turnkey Automated System
SPF-290S Sunscreen Protection Factor Analyzer System and computer comprised of a 125W CW Xenon source, integrating sphere, color-compensating filter, six sample holders, scanning monochromator with stepping motor and controller, X-Y Module and electronic X-Y Control Module, in a self-contained, attractive desktop unit. Includes data acquisition board and drivers, I/O, RS-232 and power cables and WinSPF operating software, four rolls of Transpore® tape (8-5010), a box of 25 syringes (8-5015), validation kit and complete instruction manual. WinSPF operating software also includes Audio Visual Training aids, Colipa spreadsheet, complete on-line help system, fully functional toolbar, single-step control icons, graphical and tabular data display formats and utilizes all Windows® multi-tasking features. Available for use with 110V AC, 60 Hz. (catalog no. 8-5005) or 220V AC, 50 Hz. (catalog no. 8-5006). Computer will be a current Windows® model capable of running WinSPF software and will include keyboard, monitor, mouse. Data acquisition board, drivers and Win SPF software installed and tested.
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$22,200 Annual SPF Analyzer service contract available after first year for $2,350; Contact sales for detail on contract benefits. |
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